Extraction of oospores of Peronospora viciae from soil

被引：12

作者：

vanderGaag, DJ ^{[1
]}

Frinking, HD ^{[1
]}

机构：

[1] WAGENINGEN UNIV AGR,DEPT PHYTOPATHOL,NL-6700 EE WAGENINGEN,NETHERLANDS

来源：

PLANT PATHOLOGY | 1997年 / 46卷 / 05期

关键词：

D O I：

10.1046/j.1365-3059.1997.d01-70.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A method for the extraction of oospores of Peronospora viciae from soil is described. Approximately 75% of the oospores added to silty clay loam and loamy sand soil samples were recovered. Percentage recovery was independent of oospore density. This extraction method did not affect viability as determined by the tetrazolium bromide test and a germination assay and may therefore be used to study survival of oospores. Numbers of oospores extracted from soil samples taken from seven fields naturally infested with oospores of P. viciae f.sp. pisi ranged from 2 to 21 oospores per g soil. Oospore density was not significantly correlated with disease incidence as determined by a bioassay.

引用

页码：675 / 679

页数：5

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