Serological evidence of FMD subclinical infection in sheep population during the 1999 epidemic in Morocco

被引:28
作者
Blanco, E [1 ]
Romero, LJ
El Harrach, M
Sánchez-Vizcaíno, JM
机构
[1] Minist Ciencia & Tecnol, INIA, CISA, Madrid 28130, Spain
[2] Biopharma Lab, Rabat, Morocco
关键词
FMDV; sheep; viruses; non-structural proteins; 3ABC; ELISA;
D O I
10.1016/S0378-1135(01)00473-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During 1999, 11 outbreaks of foot and mouth disease (FMD), were declared in the east and central part of Morocco. All the FMD clinical cases reported were cattle. In order to analyse them serological status of sheep from the FMD outbreak areas, 598 sheep sera were tested using a liquid-phase blocking ELISA (LPBE) to detect antibodies against FMDV structural proteins. The study confirmed the presence of FMDV specific antibodies in 77 clinically normal sheep, indicating that unrecognised FMDV-infected sheep could represent a potential risk of FMD dissemination in Morocco. Subsequently, sera from flocks of sheep that had been exposed to FMD outbreaks were assayed by an indirect ELISA using the recombinant FMDV non-structural protein 3ABC expressed in E. coli to evaluate the potential use of this serological test in future epidemiological studies and the development of FMD control strategies. The results indicated that the 3ABC-ELISA was able: to detect antibodies indicative of infection with FMDV in asymptomatic sheep in field conditions. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:13 / 21
页数:9
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