Analysis of the INK4a/ARF locus in non-Hodgkin's lymphomas using two new internal microsatellite markers

被引：17

作者：

Herranz, M

Urioste, M

Santos, J

Rivas, C

Martínez, B

Benitez, J

Fernández-Piqueras, J ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Fac Ciencias, Dept Biol, Lab Genet Mol Humana, E-28049 Madrid, Spain

[2] Fdn Jimenez Diaz, Dept Genet, E-28040 Madrid, Spain

[3] Fdn Jimenez Diaz, Dept Genet, E-28040 Madrid, Spain

[4] Fdn Jimenez Diaz, Dept Patol, E-28040 Madrid, Spain

来源：

LEUKEMIA | 1999年 / 13卷 / 05期

关键词：

INK4a/ARF locus; internal microsatellites; NHLs; LOH; genetic instability;

D O I：

10.1038/sj.leu.2401409

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Inactivation of the INK4a/ARF locus is a frequent event in non-Hodgkin's lymphomas (NHLs), which may be attributed to deletion, point mutation, and 5' CpG methylation at its promoter region. In the present study we evaluated the occurrence of deletions and genetic instability of INK4a/ARF locus in 30 paired normal and tumor samples of B cell NHLs by conducting an allelotypic analysis with two new polymorphic markers, one located at the intron 1 of p16(INK4a) gene and the other one placed downstream exon 1 beta of p19(ARF). Comparison of these results with those obtained in a previous paper using flanking markers (D9S171, D9S942, D9S956 and IFNA) allowed us to detect two new cases of microsatellite instability (L-446 and L-442), and to confirm the occurrence of LOH at the INK4a/ARF locus in one tumor (M-3770). On the contrary, this locus is not affected in three different tumors (L-421, L-272 and L-159) which exhibited LOH at some of the flanking markers.

引用

页码：808 / 810

页数：3

共 12 条

[1]

CHAUVERT P, 1997, MOL CELL PROBE, V10, P467

[2] The human protein p19(ARF) is not detected in hemopoietic human cell lines that abundantly express the alternative beta transcript of the p16(INK4a)/MTS1 gene [J].