Biological effects of the dual phenotypic Janus mutation of ret cosegregating with both multiple endocrine neoplasia type 2 and Hirschsprung's disease

被引:51
作者
Arighi, E
Popsueva, A
Degl'Innocenti, D
Borrello, MG
Carniti, C
Perälä, NM
Pierotti, MA
Sariola, H
机构
[1] Univ Helsinki, Biomedicum Helsinki, Inst Biomed, FIN-00014 Helsinki, Finland
[2] Ist Nazl Tumori, Dept Expt Oncol, I-20133 Milan, Italy
[3] Univ Helsinki, Cent Hosp, FIN-00029 Helsinki, Finland
关键词
D O I
10.1210/me.2003-0173
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Gain-of-function mutations of ret receptor tyrosine kinase, the signaling receptor for glial cell line-derived neurotrophic factor, cause sporadic thyroid and adrenal malignancies as well as endocrine cancer syndromes, such as multiple endocrine neoplasia types 2A and 2B ( MEN 2A and MEN 2B) and familial medullary thyroid carcinoma. Loss-of-function mutations of ret cause Hirschsprung's disease (HSCR) or colonic aganglionosis. In 20-30% of families with a mutation at residues 609, 611, 618, or 620 of RET, MEN 2A and familial medullary thyroid carcinoma cosegregate with HSCR. These mutations constitutively activate RET due to aberrant disulfide homodimerization and diminish the level of RET at the plasma membrane. It is not known how these mutations simultaneously lead to both gain- and loss-of-function RET-associated diseases. We provide an explanation for the dual phenotypic Janus mutation at Cys620 of RET. In Madin-Darby canine kidney (MDCK) cells, the Janus mutation impairs the glial cell line-derived neurotrophic factor-induced effects of RET on cell migration, differentiation, and survival but simultaneously promotes rapid cell proliferation.
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页码:1004 / 1017
页数:14
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