Assays for cytokines using aptamers

被引：41

作者：

Guthrie, JW ^{[1
]}

Hamula, CLA ^{[1
]}

Zhang, HQ ^{[1
]}

Le, XC ^{[1
]}

机构：

[1] Univ Alberta, Fac Med & Dent, Dept Publ Hlth Sci, Edmonton, AB T6G 2G3, Canada

来源：

METHODS | 2006年 / 38卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

aptamers; cytokines; SELEX; in vitro selection; combinatorial chemistry; proteins1;

D O I：

10.1016/j.ymeth.2006.01.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aptamers are short nucleic acid sequences that are used as ligands to bind their targets with high affinity. They are generated via the combinatorial chemistry procedure systematic evolution or ligands by exponential enrichment (SELEX). Aptamers have shown much promise towards detection of a variety of protein targets, including cytokines. Specifically, for the determination of cytokines and growth factors, several assays making use of aptainers have been developed, including aptamer-based enzyme-linked immunosorbent assays, antibody-linked oligonucleotide assay, fluorescence (anisotropy and resonance energy transfer) assays, and proximity ligation assays. In this article, the concept of aptamer selection using SELEX and the assay formats using aptamers for the 'detection of cytokines are discussed. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：324 / 330

页数：7

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