Combined assessment of genetic variability in populations of brown trout (Salmo trutta L.) based on allozymes, microsatellites, and RAPD markers

被引：32

作者：

Cagigas, ME ^{[1
]}

Vazquez, E ^{[1
]}

Blanco, G ^{[1
]}

Sánchez, JA ^{[1
]}

机构：

[1] Dept Biol Func, Area Genet, Oviedo 33071, Spain

来源：

MARINE BIOTECHNOLOGY | 1999年 / 1卷 / 03期

关键词：

Salmo trutta L; molecular markers; allozyme microsatellite; randomly amplified polymorphic DNA (RAPD);

D O I：

10.1007/PL00011778

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genetic variability within and among four Spanish natural populations of Salmo trutta L. was evaluated on the basis of 25 enzyme loci, 3 microsatellite loci, and 9 randomly amplified polymorphic DNAs (RAPDs). A total of 21 allelic markers were found, 12 of which were reported by microsatellites, whereas enzyme and RAPD accounted only for 6 and 3, respectively. Genetic variation within samples was significantly higher for microsatellites and RAPD than for enzyme loci. Although all methods reported a high degree of allelic heterogeneity among samples, also revealing a high degree of gene diversity, genetic relationships depicted by UPGMA dendrograms closely agreed for all kinds of data. Microsatellite loci appeared to be the most feasible technique when searching for specific alleles for a population or an area, owing to the higher number of allelic variants found.

引用

页码：286 / 296

页数：11

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