Monitoring the kinetics of Bacillus subtilis endospore germination via surface-enhanced Raman scattering spectroscopy

被引：45

作者：

Daniels, JK ^{[1
]}

Caldwell, TP ^{[1
]}

Christensen, KA ^{[1
]}

Chumanov, G ^{[1
]}

机构：

[1] Clemson Univ, Dept Chem, Clemson, SC 29634 USA

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 05期

关键词：

D O I：

10.1021/ac052009v

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mirror sandwich SERS substrates (M3S) were used to monitor the kinetics of Bacillus subtilis endospore germination. The sandwich configuration of the substrates allows real-time observation of germination in samples that contained only several hundred endospores. The enhancement provided by the substrates is attributed to the enhanced local electromagnetic field that originates from coupling between the Ag nanoparticles and the underlying metal film as well as from coupling between the Ag nanoparticles themselves. The germination kinetics at varying concentrations of L-alanine and different temperatures were studied by monitoring the intensity and growth of the Raman peak at 1010 cm(-1), which is characteristic of dipicolinic acid. A total of four concentrations (50, 75, 100, and 150 mM) of L-alanine and three different temperatures (30, 37, and 55 degrees C) were investigated.

引用

页码：1724 / 1729

页数：6

共 30 条

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