Sphingosylphosphorylcholine induces cytosolic Ca2+ elevation in endothelial cells in situ and causes endothelium-dependent relaxation through nitric oxide production in bovine coronary artery

被引：39

作者：

Mogami, K

Mizukami, Y

Todoroki-Ikeda, N

Ohmura, M

Yoshida, K

Miwa, S

Matsuzaki, M

Matsuda, M

Kobayashi, S

机构：

[1] Yamaguchi Univ, Sch Allied Hlth Sci, Ube, Yamaguchi 7558505, Japan

[2] Yamaguchi Univ, Sch Med, Dept Med 2, Ube, Yamaguchi 7558505, Japan

[3] Yamaguchi Univ, Sch Med, Dept Physiol 1, Ube, Yamaguchi 7558505, Japan

来源：

FEBS LETTERS | 1999年 / 457卷 / 03期

关键词：

sphingolipid; cytosolic Ca2+; nitric oxide; vasorelaxation; 2,3-diaminonaphthalene; endothelial cell;

D O I：

10.1016/S0014-5793(99)01076-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sphingosylphosphorylcholine (SPC) increased intracellular Ca2+ concentration ([Ca2+]i) and nitric oxide NO) production in endothelial cells in situ on bovine aortic valves, and induced endothelium-dependent relaxation of bovine coronary arteries precontracted with U-46619, The SPC-induced vasorelaxation was inhibited by NO-monomethyl-L-arginine, an inhibitor of both constitutive and inducible NO synthase (NOS), but not by 1-(2-trifloromethylhenyl) imidazole, an inhibitor of inducible NOS (iNOS). Immunoblotting revealed that endothelial constitutive NOS, but not iNOS, was present in endothelial cells in situ on the bovine aortic valves. We propose that SPC activates [Ca2+]i levels and NO production of endothelial cells in situ, thereby causing an endotheliumdependent vasorelaxation, (C) 1999 Federation of European Biochemical Societies.

引用

页码：375 / 380

页数：6

共 33 条

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