Purification and characterization of a new fructan series from species of Asteraceae

被引:33
作者
Ernst, M [1 ]
Chatterton, NJ [1 ]
Harrison, PA [1 ]
机构
[1] UTAH STATE UNIV,USDA ARS,LOGAN,UT 84322
关键词
Cichorium intybus; Helianthus tuberosus; Taraxacum officinale; fructan; inulo-n-ose;
D O I
10.1111/j.1469-8137.1996.tb04509.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A new fructan series, in addition to inulin, was identified in tissue extracts of Cichorium intybus L., Helianthus tuberosus L. and Taraxacum officinale Weber. The second fructan series was separated from the inulin series up to degree of polymerization (DP) 18 by ion chromatography. The DP 4 of this new fructan series was purified and structurally analysed. Hydrolysis and methylation analysis of the DP 4 oligomer shows that this is a fructan series that contains only beta-2,1-linked fructose molecules. We conclude that this new fructan series in species of Asteraceae comprises the inulo-n-ose series and probably consists of products of inulin hydrolysis that are formed during inulin mobilization.
引用
收藏
页码:63 / 66
页数:4
相关论文
共 26 条
[1]   FRUCTAN CHEMICAL-STRUCTURE AND SENSITIVITY TO AN EXOHYDROLASE [J].
BANCAL, P ;
HENSON, CA ;
GAUDILLERE, JP ;
CARPITA, NC .
CARBOHYDRATE RESEARCH, 1991, 217 :137-151
[2]   PURIFICATION AND CHARACTERIZATION OF FRUCTANS WITH BETA-2,1-GLYCOSIDIC AND BETA-2,6-GLYCOSIDIC LINKAGES SUITABLE FOR ENZYME STUDIES [J].
BONNETT, GD ;
SIMS, IM ;
STJOHN, JA ;
SIMPSON, RJ .
NEW PHYTOLOGIST, 1994, 127 (02) :261-269
[3]   SYNTHESIS OF INULIN OLIGOMERS IN TISSUE-SLICES, PROTOPLASTS AND INTACT VACUOLES OF JERUSALEM-ARTICHOKE [J].
CARPITA, NC ;
KELLER, F ;
GIBEAUT, DM ;
HOUSLEY, TL ;
MATILE, P .
JOURNAL OF PLANT PHYSIOLOGY, 1991, 138 (02) :204-210
[4]  
CHATTERTON NJ, 1989, PLANT PHYSIOL BIOCH, V27, P289
[5]   STRUCTURE OF FRUCTAN OLIGOMERS IN CHEATGRASS (BROMUS-TECTORUM L) [J].
CHATTERTON, NJ ;
HARRISON, PA ;
THORNLEY, WR ;
BENNETT, JH .
NEW PHYTOLOGIST, 1993, 124 (03) :389-396
[6]   PURIFICATION AND PROPERTIES OF AN INULINASE FROM CHICORY ROOTS (CICHORIUM-INTYBUS L) [J].
CLAESSENS, G ;
VANLAERE, A ;
DEPROFT, M .
JOURNAL OF PLANT PHYSIOLOGY, 1990, 136 (01) :35-39
[7]   LOCALIZATION OF THE ENZYMES OF FRUCTAN METABOLISM IN VACUOLES ISOLATED BY A MECHANICAL METHOD FROM TUBERS OF JERUSALEM ARTICHOKE (HELIANTHUS-TUBEROSUS L) [J].
DARWEN, CWE ;
JOHN, P .
PLANT PHYSIOLOGY, 1989, 89 (02) :658-663
[8]   MECHANISIM OF FRUCTOSAN METABOLISM IN HIGHER PLANTS AS EXEMPLIFIED IN HELIANTHUS TUBEROSUS [J].
EDELMAN, J ;
JEFFORD, TG .
NEW PHYTOLOGIST, 1968, 67 (03) :517-+
[9]   METABOLISM OF FRUCTOSE POLYMERS IN PLANTS .4. BETA-FRUCTOFURANOSIDASES OF TUBERS OF HELIANTHUS TUBEROSUS L [J].
EDELMAN, J ;
JEFFORD, TG .
BIOCHEMICAL JOURNAL, 1964, 93 (01) :148-+
[10]   GLUCOFRUCTOSAN METABOLISM IN CICHORIUM-INTYBUS ROOTS [J].
GUPTA, AK ;
MAMTA ;
BHATIA, IS .
PHYTOCHEMISTRY, 1985, 24 (07) :1423-1427