A 24-hour screening protocol for identification of vancomycin-resistant Enterococcus faecium

被引:17
作者
Drews, SJ
Johnson, G
Gharabaghi, F
Roscoe, M
Matlow, A
Tellier, R
Richardson, SE
机构
[1] Hosp Sick Children, Dept Paediat, Toronto, ON M5G 1X8, Canada
[2] Hosp Sick Children, Div Microbiol, Toronto, ON M5G 1X8, Canada
[3] Univ Toronto, Dept Pathobiol & Lab Med, Toronto, ON, Canada
关键词
D O I
10.1128/JCM.44.4.1578-1580.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We describe a 24-h protocol for the identification of patients who are positive for vancomycin-resistant Enterococcus faecium (VRE), using stool and rectal swab samples and VRE screening broth, automated DNA extraction, and real-time PCR for vanA and vanB genes. Compared to conventional screening methods, this protocol had a high sensitivity and specificity and a negative predictive value.
引用
收藏
页码:1578 / 1580
页数:3
相关论文
共 12 条
[1]  
Blijlevens NMA, 2005, CURR OPIN ONCOL, V17, P605
[2]   EPIDEMIOLOGIC ANALYSIS AND GENOTYPIC CHARACTERIZATION OF A NOSOCOMIAL OUTBREAK OF VANCOMYCIN-RESISTANT ENTEROCOCCI [J].
BOYLE, JF ;
SOUMAKIS, SA ;
RENDO, A ;
HERRINGTON, JA ;
GIANARKIS, DG ;
THURBERG, BE ;
PAINTER, BG .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (05) :1280-1285
[3]   IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM HUMAN INFECTIONS BY A CONVENTIONAL TEST SCHEME [J].
FACKLAM, RR ;
COLLINS, MD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (04) :731-734
[4]   EMERGENCE OF VANCOMYCIN-RESISTANT ENTEROCOCCI IN NEW-YORK-CITY [J].
FRIEDEN, TR ;
MUNSIFF, SS ;
LOW, DE ;
WILLEY, BM ;
WILLIAMS, G ;
FAUR, Y ;
EISNER, W ;
WARREN, S ;
KREISWIRTH, B .
LANCET, 1993, 342 (8863) :76-79
[5]  
Hospital Infection Control Practices Advisory Committee (HICPAC), 1995, INFECT CONT HOSP EP, V16, P105
[6]   Clinical prevalence, antimicrobial susceptibility, and geographic resistance patterns of enterococci: Results from the SENTRY Antimicrobial Surveillance Program, 1997-1999 [J].
Low, DE ;
Keller, N ;
Barth, A ;
Jones, RN .
CLINICAL INFECTIOUS DISEASES, 2001, 32 :S133-S145
[7]  
McGeer A J, 2000, Semin Respir Infect, V15, P314
[8]   Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis [J].
Morata, P ;
Queipo-Ortuño, MI ;
Colmenero, JD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) :2443-2446
[9]   Rapid detection of vanA and vanB genes directly from clinical specimens and enrichment broths by real-time multiplex PCR assay [J].
Palladino, S ;
Kay, ID ;
Flexman, JP ;
Boehm, I ;
Costa, AMG ;
Lambert, EJ ;
Christiansen, KJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (06) :2483-2486
[10]   Management of bleeding in the terminally ill patient [J].
Prommer, E .
HEMATOLOGY, 2005, 10 (03) :167-175