Antisense transgenics in animals

被引：17

作者：

Erickson, RP ^{[1
]}

机构：

[1] Univ Arizona, Coll Med,Sect Med & Mol Genet, Angel Char Children Wings Genet Res, Steele Mem Childrens Res Ctr,Dept Pediat, Tucson, AZ 85724 USA

来源：

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1999年 / 18卷 / 03期

关键词：

D O I：

10.1006/meth.1999.0788

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antisense transgenesis provides a methodology for ablating gene expression in targeted tissues through the use of tissue-specific or controllable promoters. The two major features to be considered in the design of a construct for injection are (1) the target sequence and (2) the promoter to be used, information is provided to help the investigator make decisions in these regards. The standard methodology of making transgenics is not replicated but some hints as how best to use a transgenic facility are provided. An overview of methods for studying transgene expression and target gene suppression is given. In summary, antisense transgenesis may provide a more readily achievable method for tissue-specific ablation of a gene's function than controllable knockouts provide. (C) 1999 Academic Press.

引用

页码：304 / 310

页数：7

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