Molecular cloning and tissue-specific, developmental-stage-specific, and hormonal regulation of IGFBP3 gene in zebrafish

被引:77
作者
Chen, JY
Chen, JC
Huang, WT
Liu, CW
Hui, CF
Chen, TT
Wu, JL [1 ]
机构
[1] Acad Sinica, Inst Zool, Taipei, Taiwan
[2] So Taiwan Univ Technol, Dept Chem Engn, Tainan, Taiwan
[3] Dayeh Univ, Inst Agr Biotechnol, Da Tsuen, Chang Hwa, Taiwan
[4] Univ Connecticut, Dept Mol & Cell Biol, Storrs, CT 06269 USA
关键词
cloning; zebrafish; IGFBP3; hormone regulation;
D O I
10.1007/s10126-002-0115-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The biological functions of insulin-like growth factor (IGF) I and II are modulated by a family of IGF-binding proteins (IGFBPs) in complex IGF-dependent and IGF-independent pathways. For further understanding of the actions of IGFs, some of these binding proteins have been cloned and characterized. We report the molecular cloning of IGFBP-3 cDNA for zebrafish. The tissue-specific and developmental stage-specific expression of IGFBP-3 and the hormonal regulation of its expression have also been determined by comparative reverse transcription polymerase chain reaction. Zebrafish IGFBP-3 cDNA contains an open reading frame of 879 bp, encoding a polypeptide of 293 amino acid residues. Results of this analysis revealed high levels of IGFBP-3 messenger RNA in ovary and fin tissue. Expression of IGFBP-3 mRNA was throughout the entire embryonic development, with the highest level of expression observed at 36 hours after the onset of development. Elevated levels of expression of IGFBP-3 were observed 24 hours after injection with IGF-I and 48 hours with IGF-II or insulin. These results suggest that the expression of IGFBP3 gene might be modulated by IGF-I, IGF-II, and insulin.
引用
收藏
页码:1 / 7
页数:7
相关论文
共 25 条
[1]   Insulin-like growth factor (IGF)-binding proteins: interactions with IGFs and intrinsic bioactivities [J].
Baxter, RC .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2000, 278 (06) :E967-E976
[2]   Production of biologically active recombinant tilapia insulin-like growth factor-II polypeptides in Escherichia coli cells and characterization of the genomic structure of the coding region [J].
Chen, JY ;
Chang, CY ;
Chen, JC ;
Shen, SC ;
Wu, JL .
DNA AND CELL BIOLOGY, 1997, 16 (07) :883-892
[3]   Isolation and characterization of Tilapia (Oreochromis mossambicus) insulin-like growth factors gene and proximal promoter region [J].
Chen, JY ;
Tsai, HL ;
Chang, CY ;
Wang, JI ;
Shen, SC ;
Wu, JL .
DNA AND CELL BIOLOGY, 1998, 17 (04) :359-376
[4]   Expression of recombinant tilapia insulin-like growth factor-I and stimulation of juvenile tilapia growth by injection of recombinant IGFs polypeptides [J].
Chen, JY ;
Chen, JC ;
Chang, CY ;
Shen, SC ;
Chen, MS ;
Wu, JL .
AQUACULTURE, 2000, 181 (3-4) :347-360
[5]   Insulin-like growth factor binding protein 2 is a growth inhibitory protein conserved in zebrafish [J].
Duan, CM ;
Ding, J ;
Li, Q ;
Tsai, W ;
Pozios, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :15274-15279
[6]  
Ferry RJ, 1999, HORM RES, V51, P53
[7]   Cellular actions of insulin-like growth factor binding proteins [J].
Ferry, RJ ;
Katz, LEL ;
Grimberg, A ;
Cohen, P ;
Weinzimer, SA .
HORMONE AND METABOLIC RESEARCH, 1999, 31 (2-3) :192-202
[8]  
Flint DJ, 2001, LIVEST PROD SCI, V70, P115, DOI 10.1016/S0301-6226(01)00203-2
[9]   Developmental changes in serum levels of free and total insulin-like growth factor I (IGF-I), IGF-Binding protein-1 and -3, and the acid-labile subunit in rats [J].
Frystyk, J ;
Gronbæk, H ;
Skjærbæk, C ;
Flyvbjerg, A ;
Orskov, H ;
Baxter, RC .
ENDOCRINOLOGY, 1998, 139 (10) :4286-4292
[10]  
Greene MW, 1999, MOL REPROD DEV, V54, P348, DOI 10.1002/(SICI)1098-2795(199912)54:4&lt