Molecularly imprinted ligand-exchange recognition assay of DNA by SPR system using guanosine and guanine recognition sites of DNA

被引：30

作者：

Diltemiz, S. Emir ^{[1
]}

Denizli, Adil ^{[3
]}

Ersoez, Arzu

Say, Ridvan ^{[2
]}

机构：

[1] Anadolu Univ, Fen Fak, Dept Chem, TR-26470 Eskisehir, Turkey

[2] Anadolu Univ, BIBAM Plant Drug & Sci Res Ctr, TR-26470 Eskisehir, Turkey

[3] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2008年 / 133卷 / 02期

关键词：

molecular imprinting polymers(MIPs); surface plasmon resonance (SPR); guanosine; guanine; ligand exchange (LE);

D O I：

10.1016/j.snb.2008.03.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

Molecular imprinting polymer (MIP) as a recognition element for sensors are increasingly of interest and MIP-SPR have started to appear in the literature. In this Study, we have proposed a novel surface imprinting method for DNA recognition and determination by methacryl-amidohistidine-platinum(II) [MAH-Pt(II)] as a new metal-chelating monomer via metal coordination-chelation interactions and guanosine/guanine templates of DNA. The study includes the measurement of binding interaction of molecular imprinted Surface plasmon resonance (SPR) sensor via ligand interaction and recognition selectivity studies of guanosine and guanine imprinted polymers with respect to guanine and dsDNA. Adsorption isotherms of adenosine, guanosine, guanine, adenine, ssDNA and dsDNA were plotted and affinity constants for guanosine and guanine imprinted films were also calculated. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：484 / 488

页数：5

共 32 条

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