Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library

被引：90

作者：

Li, R

Mignot, E

Faraco, J

Kadotani, H

Cantanese, J

Zhao, B

Lin, X

Hinton, L

Ostrander, EA

Patterson, DF

de Jong, PJ

机构：

[1] Stanford Ctr Narcolepsy Res, Stanford, CA 94305 USA

[2] Roswell Pk Canc Inst, Dept Canc Genet, Buffalo, NY 14263 USA

[3] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA

[4] Univ Penn, Sch Vet Med, Med Genet Sect, Philadelphia, PA 19104 USA

来源：

GENOMICS | 1999年 / 58卷 / 01期

关键词：

D O I：

10.1006/geno.1999.5772

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A large insert canine genomic bacterial artificial chromosome (BAC) library was built from a Doberman pinscher. Approximately 166,000 clones were gridded on nine high-density hybridization filters. Insert analysis of randomly selected clones indicated a mean insert size of 155 kb and predicted 8.1 coverage of the canine genome. Two percent of the clones were nonrecombinant. Chromosomal fluorescence in situ hybridization studies of 60 BAC clones indicated no chimerism. The library was hybridized with dog PCR products representing eight genes (ADA, TNFA, GCA, MYB, HOXA, GUSB, THY1, and TOP1). The resulting positive clones were characterized and shown to be compatible with an eightfold redundant library. (C) 1999 Academic Press.

引用

页码：9 / 17

页数：9

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