Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens

被引：36

作者：

Halliday, C ^{[1
]}

Wu, QX

James, G

Sorrell, T

机构：

[1] Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

[2] Univ Sydney, Western Clin Sch, NHMRC Clin Ctr Res Excellence Infect Dis Hematol, Westmead, NSW 2145, Australia

[3] Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 10期

关键词：

D O I：

10.1128/JCM.43.10.5366-5368.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a LightCycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.

引用

页码：5366 / 5368

页数：3

共 13 条

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