Concentration of GPI-Anchored Proteins upon ER Exit in Yeast

被引：141

作者：

Castillon, Guillaume A. ^{[1
]}

Watanabe, Reika ^{[1
]}

Taylor, Marcia ^{[1
]}

Schwabe, Tatjana M. E. ^{[1
]}

Riezman, Howard ^{[1
]}

机构：

[1] Univ Geneva, Dept Biochem, CH-1211 Geneva, Switzerland

来源：

TRAFFIC | 2009年 / 10卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

Bst1p; COPII; Cwp2p; Emp24p; ER exit site; Erv14p; GPI-anchored proteins; Hxt1p; Per1p; ENDOPLASMIC-RETICULUM; SACCHAROMYCES-CEREVISIAE; FLUORESCENT PROTEIN; TRANSPORT VESICLES; TRANSMEMBRANE PROTEIN; MEMBRANE-PROTEINS; SECRETORY PROTEIN; COATED VESICLES; QUALITY-CONTROL; GOLGI-COMPLEX;

D O I：

10.1111/j.1600-0854.2008.00857.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Previous biochemical work has revealed two parallel routes of exit from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae, one seemingly specific for glycosyl-phosphatidylinositol (GPI)-anchored proteins. Using the coat protein II (COPII) mutant sec31-1, we visualized ER exit sites (ERES) and identified three distinct ERES populations in vivo. One contains glycosylated pro-alpha-factor, the second contains the GPI-anchored proteins Cwp2p, Ccw14p and Tos6p and the third is enriched with the hexose transporter, Hxt1p. Concentration of GPI-anchored proteins prior to budding requires anchor remodeling, and Hxt1p incorporation into ERES requires the COPII components Sec12p and Sec16p. Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p.

引用

页码：186 / 200

页数：15

共 55 条

[1] The organization of endoplasmic reticulum export complexes [J].

Bannykh, SI ;

Rowe, T ;

Balch, WE .

JOURNAL OF CELL BIOLOGY, 1996, 135 (01) :19-35

[2] COPII - A MEMBRANE COAT FORMED BY SEC PROTEINS THAT DRIVE VESICLE BUDDING FROM THE ENDOPLASMIC-RETICULUM [J].

BARLOWE, C ;

ORCI, L ;

YEUNG, T ;

HOSOBUCHI, M ;

HAMAMOTO, S ;

SALAMA, N ;

REXACH, MF ;

RAVAZZOLA, M ;

AMHERDT, M ;

SCHEKMAN, R .

CELL, 1994, 77 (06) :895-907

[3] SEC12 ENCODES A GUANINE-NUCLEOTIDE-EXCHANGE FACTOR ESSENTIAL FOR TRANSPORT VESICLE BUDDING FROM THE ER [J].

BARLOWE, C ;

SCHEKMAN, R .

NATURE, 1993, 365 (6444) :347-349

[4] Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins [J].

Barz, WP ;

Walter, P .

MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (04) :1043-1059

[5] Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex [J].

Belden, WJ ;

Barlowe, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (46) :43040-43048

[6] An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast [J].

Bellí, G ;

Garí, E ;

Piedrafita, L ;

Aldea, N ;

Herrero, E .

NUCLEIC ACIDS RESEARCH, 1998, 26 (04) :942-947

[7] Drosophila Cornichon acts as cargo receptor for ER export of the TGFα-like growth factor Gurken [J].

Bökel, C ;

Dass, S ;

Wilsch-Bräuninger, M ;

Roth, S .

DEVELOPMENT, 2006, 133 (03) :459-470

[8] GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor [J].

Bosson, Regine ;

Jaquenoud, Malika ;

Conzelmann, Andreas .

MOLECULAR BIOLOGY OF THE CELL, 2006, 17 (06) :2636-2645

[9] Sec16 is a determinant of transitional ER organization [J].

Connerly, PL ;

Esaki, M ;

Montegna, EA ;

Strongin, DE ;

Levi, S ;

Soderholm, J ;

Glick, BS .

CURRENT BIOLOGY, 2005, 15 (16) :1439-1447

[10] Genome-wide identification of fungal GPI proteins [J].

de Groot, PWJ ;

Hellingwerf, KJ ;

Klis, FM .

YEAST, 2003, 20 (09) :781-796

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