Enhancement of the binding ability of a ligand for nucleobase recognition by introducing a methyl group

被引：48

作者：

Dai, Q

Xu, CY

Sato, Y

Yoshimoto, K

Nishizawa, S

Teramae, N ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Sci, Dept Chem, Sendai, Miyagi 9808578, Japan

[2] JST, CREST, Aoba Ku, Sendai, Miyagi 9808578, Japan

[3] RIKEN, Inst Phys & Chem Res, Bioengn Lab, Wako, Saitama 3510198, Japan

来源：

ANALYTICAL SCIENCES | 2006年 / 22卷 / 02期

关键词：

D O I：

10.2116/analsci.22.201

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5 degrees C; 6.2 x 10(6) M-1 of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteri dine and guanine opposite the AP site in water.

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收藏

页码：201 / 203

页数：3

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