ProbeDesigner for the design of probesets for branched DNA (bDNA) signal amplification assays

被引：27

作者：

Bushnell, S ^{[1
]}

Budde, J ^{[1
]}

Catino, T ^{[1
]}

Cole, J ^{[1
]}

Derti, A ^{[1
]}

Kelso, R ^{[1
]}

Collins, ML ^{[1
]}

Molino, G ^{[1
]}

Sheridan, P ^{[1
]}

Monahan, J ^{[1
]}

Urdea, M ^{[1
]}

机构：

[1] Chiron Diagnost, Walpole, MA 02032 USA

来源：

BIOINFORMATICS | 1999年 / 15卷 / 05期

关键词：

D O I：

10.1093/bioinformatics/15.5.348

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Motivation: The sensitivity and specificity of branched DNA (bDNA) assays are derived in part through the judicious design of the capture and label extender probes. To minimize non-specific hybridization (NSH) events, which elevate assay background, candidate probes must be computer screened fro complementarity with generic sequences present in the assay. Results: We present a software application which allows for rapid and flexible design of bDNA probesets for novel targets. It includes an algorithm for estimating the magnitude of NSH contribution to background, a mechanism for removing probes with elevated contributions, a methodology for the simultaneous design of probesets for multiple targets, and a graphical user interface which guides the user through the design steps.

引用

页码：348 / 355

页数：8

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