DETERMINATION OF ZEARALENONE BY THE CAPILLARY ZONE ELECTROPHORESIS-UV DETECTION AND ITS APPLICATION TO POULTRY FEED AND CEREALS

被引：4

作者：

Guray, Tufan ^{[1
]}

Tuncel, Muzaffer ^{[2
]}

Uysal, Ulku Dilek ^{[3
]}

Oncu-Kaya, Elif Mine ^{[3
]}

机构：

[1] Eskisehir Osmangazi Univ, Fac Arts & Sci, Dept Chem, TR-26480 Eskisehir, Turkey

[2] Anadolu Univ, Dept Analyt Chem, Fac Pharm, Eskisehir, Turkey

[3] Anadolu Univ, Dept Chem, Fac Sci, Eskisehir, Turkey

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2013年 / 36卷 / 10期

关键词：

capillary electrophoresis; grain; maize flour; mycotoxin; poultry feed; zearalenone; PERFORMANCE LIQUID-CHROMATOGRAPHY; MACROCYCLIC LACTONE MYCOTOXINS; TANDEM MASS-SPECTROMETRY; GAS-CHROMATOGRAPHY; FUSARIUM TOXINS; ANIMAL FEED; BABY FOOD; OCHRATOXIN; CORN; AFLATOXINS;

D O I：

10.1080/10826076.2012.691437

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary zone electrophoretic method for the determination of zearalenone is described in this study. In the separation, a run buffer consisting of 20mM sodium tetraborate at pH 9.0 with 15% acetonitrile applying 15kV, injecting 10s at 50 mbar was utilized. Phenobarbital was a suitable internal standard and the signals were recorded at 254nm. Zearalanon and internal standard appeared at 5.46min (RSD% 0.20) and 6.35min (RSD% 0.18), respectively. The repeatability results are in the range of 0.011.58 for inter-day. The linearity was investigated in the range of 5.20x107M to 7.86x105M ZEA and it was linear fitting to the equation of [rPN=244803.8 C(M) 0.0348; r=0.9999]. The LOD and LOQ were calculated to be 2.70x108M (8.25 mu g/L) and 8.17x108M (25 mu g/L), respectively. Then, the method was successfully applied to the analysis of ZEA in poultry feeds, flour of maize, grain, and certain cereal samples such as fibrous biscuit, popcorn, and rice crisp.

引用

页码：1366 / 1378

页数：13

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