The behaviors of Ca2+-ATPase embedded in interdigitated bilayer

被引：7

作者：

Lu, JZ ^{[1
]}

Huang, F ^{[1
]}

Chen, JW ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

JOURNAL OF BIOCHEMISTRY | 1999年 / 126卷 / 02期

关键词：

Ca2+-ATPase; interdigitated bilayer; lysophosphatidylcholine;

D O I：

10.1093/oxfordjournals.jbchem.a022449

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the behavior of a membrane protein, Ca2+-ATPase, in interdigitated phospholipid bilayers, The results showed that Ca2+-ATPase does not cause significant alterations in the interdigitation of 16:0 LPC/DPPC (27.0 mol% LPC) vesicles when it is reconstituted with lipids. Intrinsic fluorescence, acrylodan fluorescent adducts, and CD spectra indicated that Ca2+-ATPase, when embedded in interdigitated bilayer structures, is more exposed to the hydrophilic environment and has a looser structure than when embedded in non-interdigitated bilayers, The interdigitation of acyl chains induces a rapid loss of enzyme activity, It is suggested that interdigitated bilayer structures may play an important role as negative regulatory factors in physiological functions.

引用

页码：302 / 306

页数：5

共 36 条

[1] ROLE OF LYSOPHOSPHATIDYLCHOLINE IN LYMPHOCYTE-T ACTIVATION - INVOLVEMENT OF PHOSPHOLIPASE-A2 IN SIGNAL TRANSDUCTION THROUGH PROTEIN-KINASE-C [J].

ASAOKA, Y ;

OKA, M ;

YOSHIDA, K ;

SASAKI, Y ;

NISHIZUKA, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6447-6451

[2] EFFECTS OF LIPID ENVIRONMENT ON THE LIGHT-INDUCED CONFORMATIONAL-CHANGES OF RHODOPSIN .1. ABSENCE OF METARHODOPSIN-II PRODUCTION IN DIMYRISTOYLPHOSPHATIDYLCHOLINE RECOMBINANT MEMBRANES [J].

BALDWIN, PA ;

HUBBELL, WL .

BIOCHEMISTRY, 1985, 24 (11) :2624-2632

[3]

BIGGS JM, 1981, BIOCHEMISTRY-US, V20, P6066

[4] DO THE LONG FATTY-ACID CHAINS OF SPHINGOLIPIDS INTERDIGITATE ACROSS THE CENTER OF A BILAYER OF SHORTER CHAIN SYMMETRICAL PHOSPHOLIPIDS [J].

BOGGS, JM ;

KOSHY, KM .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1994, 1189 (02) :233-241

[5] 2 CA-2+ ATPASE GENES - HOMOLOGIES AND MECHANISTIC IMPLICATIONS OF DEDUCED AMINO-ACID-SEQUENCES [J].

BRANDL, CJ ;

GREEN, NM ;

KORCZAK, B ;

MACLENNAN, DH .

CELL, 1986, 44 (04) :597-607

[6]

Brown RE, 1998, J CELL SCI, V111, P1

[7] Effects of anisodamine on the phase behavior of DPPG/DMPC binary mixtures [J].

Chen, JW ;

Liu, FS ;

Hwang, F .

CHEMISTRY AND PHYSICS OF LIPIDS, 1998, 91 (02) :119-127

[8] GRAMICIDIN-A SHORT-CHAIN PHOSPHOLIPID DISPERSIONS - CHAIN-LENGTH DEPENDENCE OF GRAMICIDIN CONFORMATION AND LIPID ORGANIZATION [J].

GREATHOUSE, DV ;

HINTON, JF ;

KIM, KS ;

KOEPPE, RE .

BIOCHEMISTRY, 1994, 33 (14) :4291-4299

[9] COMPUTED CIRCULAR DICHROISM SPECTRA FOR EVALUATION OF PROTEIN CONFORMATION [J].

GREENFIE.N ;

FASMAN, GD .

BIOCHEMISTRY, 1969, 8 (10) :4108-&

[10] A drug-lipid interaction model: Atropine induces interdigitated bilayer structure [J].

Hao, YH ;

Xu, YM ;

Chen, JW ;

Huang, F .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 245 (02) :439-442

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