DNA End Resection: Nucleases Team Up with the Right Partners to Initiate Homologous Recombination

被引：145

作者：

Cejka, Petr ^{[1
]}

机构：

[1] Univ Zurich, Inst Mol Canc Res, CH-8057 Zurich, Switzerland

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2015年 / 290卷 / 38期

基金：

瑞士国家科学基金会;

关键词：

DOUBLE-STRAND-BREAK; SACCHAROMYCES-CEREVISIAE; RECBCD ENZYME; CHECKPOINT ACTIVATION; GENOME STABILITY; DAMAGE RESPONSE; MRE11; NUCLEASE; FUNCTIONAL MRX; BUDDING YEAST; EXONUCLEASE;

D O I：

10.1074/jbc.R115.675942

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The repair of DNA double-strand breaks by homologous recombination commences by nucleolytic degradation of the 5'-terminated strand of the DNA break. This leads to the formation of 3'-tailed DNA, which serves as a substrate for the strand exchange protein Rad51. The nucleoprotein filament then invades homologous DNA to drive template-directed repair. In this review, I discuss mainly the mechanisms of DNA end resection in Saccharomyces cerevisiae, which includes short-range resection by Mre11-Rad50-Xrs2 and Sae2, as well as processive long-range resection by Sgs1-Dna2 or Exo1 pathways. Resection mechanisms are highly conserved between yeast and humans, and analogous machineries are found in prokaryotes as well.

引用

页码：22931 / 22938

页数：8

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