Linking SNPs to CAG repeat length in Huntington's disease patients

被引：27

作者：

Liu, Wanzhao ^{[3
]}

Kennington, Lori A. ^{[3
]}

Rosas, H. Diana ^{[4
]}

Hersch, Steven ^{[4
]}

Cha, Jang-Ho ^{[4
]}

Zamore, Phillip D. ^{[1
,2
]}

Aronin, Neil ^{[3
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Mol Pharmacol & Biochem, Worcester, MA 01605 USA

[2] Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01605 USA

[3] Univ Massachusetts, Sch Med, Dept Med, Worcester, MA 01605 USA

[4] Massachusetts Gen Hosp, MassGen Inst Neurodegenerat Dis, Charlestown, MA 02129 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 11期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth.1261

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Allele-specific silencing using small interfering RNAs targeting heterozygous single-nucleotide polymorphisms (SNPs) is a promising therapy for human trinucleotide repeat diseases such as Huntington's disease. Linking SNP identities to the two HTT alleles, normal and disease-causing, is a prerequisite for allele-specific RNA interference. Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG repeat length and nucleotide identity of heterozygous SNPs using Huntington's disease patient peripheral blood samples.

引用

页码：951 / 953

页数：3

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