Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage

被引:103
作者
Xu, WJ
Mitchell, AP
机构
[1] Columbia Univ, Dept Microbiol, Integrated Program Cellular Mol & Biophys Studies, New York, NY 10032 USA
[2] Columbia Univ, Inst Canc Res, New York, NY 10032 USA
关键词
D O I
10.1128/JB.183.23.6917-6923.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Saccharomyces cerevisiae zinc finger protein Rim101p is activated by cleavage of its C-terminal region, which resembles PEST regions that confer susceptibility to proteolysis. Here we report that Rim20p, a member of the broadly conserved PalA/AIP1/Alix family, is,required for Rim101p cleavage. Two-hybrid and coimmunoprecipitation assays indicate that Rim20p binds to Rim101p, and a two-hybrid assay shows that the Rim101p PEST-like region is sufficient for Rim20p binding. Rim101p-Rim20p interaction is conserved in Candida albicans, supporting the idea that interaction is functionally significant. Analysis of Rim20p mutant proteins indicates that some of its broadly conserved regions are required for processing of Rim101p and for stability of Rim20p itself but are not required for interaction with Rim101p. A recent genome-wide two-hybrid study (T. Ito, T. Chiba, R. Ozawa, M. Yoshida, M., Hattori, and Y. Sakaki, Proc. Nad. Acad. Sci. USA 98:4569-4574,2000) indicates that Rim20p interacts with Snf7p and that Snf7p interacts with Rim13p, a cysteine protease required for Rim101p proteolysis. We suggest that Rim20p may serve as part of a scaffold that places Rim101p and Rim13p in close proximity.
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页码:6917 / 6923
页数:7
相关论文
共 54 条
[1]  
[Anonymous], 1994, METHODS YEAST GENETI
[2]   The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function [J].
Babst, M ;
Wendland, B ;
Estepa, EJ ;
Emr, SD .
EMBO JOURNAL, 1998, 17 (11) :2982-2993
[3]   The ubiquitin-proteasome pathway: on protein death and cell life [J].
Ciechanover, A .
EMBO JOURNAL, 1998, 17 (24) :7151-7160
[4]   RIM101-dependent and -independent pathways govern pH responses in Candida albicans [J].
Davis, D ;
Wilson, RB ;
Mitchell, AP .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (03) :971-978
[5]   The proteasome, a novel protease regulated by multiple mechanisms [J].
DeMartino, GN ;
Slaughter, CA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (32) :22123-22126
[6]   SIGNALING OF AMBIENT PH IN ASPERGILLUS INVOLVES A CYSTEINE PROTEASE [J].
DENISON, SH ;
OREJAS, M ;
ARST, HN .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (48) :28519-28522
[7]   Putative membrane components of signal transduction pathways for ambient pH regulation in Aspergillus and meiosis in Saccharomyces are homologous [J].
Denison, SH ;
Negrete-Urtasun, S ;
Mingot, JR ;
Tilburn, J ;
Mayer, WA ;
Goel, A ;
Espeso, EA ;
Peñalva, MA ;
Arst, N .
MOLECULAR MICROBIOLOGY, 1998, 30 (02) :259-264
[8]   Endocytosis and signaling: An inseparable partnership [J].
Di Fiore, PP ;
De Camilli, P .
CELL, 2001, 106 (01) :1-4
[9]   THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT [J].
DURFEE, T ;
BECHERER, K ;
CHEN, PL ;
YEH, SH ;
YANG, YZ ;
KILBURN, AE ;
LEE, WH ;
ELLEDGE, SJ .
GENES & DEVELOPMENT, 1993, 7 (04) :555-569
[10]   Pheromone response, mating and cell biology [J].
Elion, EA .
CURRENT OPINION IN MICROBIOLOGY, 2000, 3 (06) :573-581