Antifungal biflavones from Cupressocyparis leylandii

From the leaves of Cupressocyparis leylandii (Cupressaceae) cupressuflavone, 4'-O-methylcupressuflavone, amentoflavone, 7-O-methylamentoflavone, 4"'-O-methylamentoflavone and hinokiflavone were isolated. H-1- and C-13-NMR data for 4'-O-methylcupressuflavone are given for the first time. The biflavones from cultivar varieties of C. leylandii (Naylor's Blue, Castlewellan Gold) were chromatographically (HPLC) compared. The antifungal activity of cupressuflavone and 4'-O-methylcupressuflavone against Alternaria alternata, Cladosporium oxysporum, Fusarium culmorum and F. avenaceum was assayed.