Rapid determination of fumonisin B1 in food samples by enzyme-linked Immunosorbent assay and colloidal gold immunoassay

被引：126

作者：

Wang, S ^{[1
]}

Quan, Y

Lee, N

Kennedy, IR

机构：

[1] Tianjin Univ Sci & Technol, Tianjin Key Lab Food Nutr & Safety, Fac Food Engn & Biotechnol, Tianjin 300222, Peoples R China

[2] Univ New S Wales, Dept Food Sci, Sydney, NSW 2052, Australia

[3] Univ Sydney, Fac Agr Food & Nat Resources, Sydney, NSW 2006, Australia

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 07期

关键词：

fumonisin; B-1; rapid microwell plate ELISA; colloidal gold immunoassay; on-site screening;

D O I：

10.1021/jf0530401

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A rapid enzyme-linked immunosorbent assay (ELISA) test (microwell plate) and a membrane-based colloidal gold immunoassay in flow-through and lateral-flow formats for the rapid detection of fumonisin B, (FBI) were developed. The rapid microwell assay can be completed within 20 min with the detection limit of 0.5 +/- 0.2 mu g/L. Membrane-based colloidal gold immunoassays had a visual detection limit of 1.0 mu g/L for FBI with the detection time of < 10 min, Matrix interference was eliminated by 15-fold dilutions of methanol extracts with buffer. These immunoassays can be used as quantitative or qualitative tools for the rapid detection of FB1 residues in 10-20 min on-site.

引用

页码：2491 / 2495

页数：5

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