Production of recombinant proteins by high cell density culture of Escherichia coli

被引：234

作者：

Choi, JH

Keum, KC

Lee, SY

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Metab & Biomol Engn Res Lab,BioProc Engn Res Ctr, Dept Biosyst,Bioinformat Res Ctr, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Ctr Ultramicrochem Proc Syst, Taejon 305701, South Korea

[3] Yonsei Univ, Coll Med, Dept Radiat Oncol, Seoul 120752, South Korea

来源：

CHEMICAL ENGINEERING SCIENCE | 2006年 / 61卷 / 03期

关键词：

high cell density culture; transcriptomics; proteomics; systems biology; Escherichia coli; recombinant protein;

D O I：

10.1016/j.ces.2005.03.031

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Escherichia coli has been the most widely used host for the production of recombinant proteins because it is the best characterized system in every aspect. Furthermore, the high cell density culture of recombinant E. coli has allowed production of various proteins with high yield and high productivities. Various cultivation strategies employing different host strains and expression systems have been successfully employed for the production of recombinant proteins. New strategies for strain improvement towards the goal of enhanced protein production are actively being developed based on high-throughput omics approaches such as transcriptomics and proteomcs. This paper reviews recent advances in the production of recombinant proteins by high cell density culture of E. coli. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：876 / 885

页数：10

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