Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination

被引:20
作者
Elder, RT
Song, XQ
Chen, MZ
Hopkins, KM
Lieberman, HB
Zhao, YQ
机构
[1] Northwestern Univ, Sch Med, Childrens Mem Inst Educ & Res, Dept Pediat, Chicago, IL 60614 USA
[2] Northwestern Univ, Sch Med, Childrens Mem Inst Educ & Res, Dept Microbiol Immunol, Chicago, IL 60614 USA
[3] Columbia Univ Coll Phys & Surg, Ctr Radiol Res, New York, NY 10032 USA
关键词
D O I
10.1093/nar/30.2.581
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A functional homolog (rhp23) of human HHR23A and Saccharomyces cerevisiae RAD23 was cloned from the fission yeast Schizosaccharomyces pombe and characterized. Consistent with the role of Rad23 homologs in nucleotide excision repair, rhp23 mutant cells are moderately sensitive to UV light but demonstrate wild-type resistance to x-rays and hydroxyurea. Expression of the rhp23, RAD23 or HHR23A cDNA restores UV resistance to the mutant, indicating that rhp23 is a functional homolog of the human and S. cerevisiae genes. The rhp23::ura4 mutation also causes a delay in the G(2) phase of the cell cycle which is corrected when rhp23, RAD23 or HHR23A cDNA is expressed. Rhp23 is present throughout the cell but is located predominantly in the nucleus, and the nuclear levels of Rhp23 decrease around the time of S phase in the cell cycle. Rhp23 is ubiquitinated at low levels, but overexpression of the rhp23 cDNA induces a large increase in ubiquitination of other proteins. Consistent with a role in protein ubiquitination, Rhp23 binds ubiquitin, as determined by two-hybrid analysis. Thus, the rhp23 gene plays a role not only in nucleotide excision repair but also in cell cycle regulation and the ubiquitination pathways.
引用
收藏
页码:581 / 591
页数:11
相关论文
共 50 条
[1]  
ALFA C, 1993, EXPT FISSION YEAST L, P19
[2]  
ALFA C, 1993, EXPT FISSION YEAST L, P52
[3]  
ALFA C, 1993, EXPT FISSION YEAST L, P1
[4]  
ALFA CE, 1990, J CELL SCI, V96, P71
[5]  
[Anonymous], METHOD ENZYMOL
[6]  
BEMEBURG M, 2001, ADV GENET, V43, P71
[7]   UBA domains of DNA damage-inducible proteins interact with ubiquitin [J].
Bertolaet, BL ;
Clarke, DJ ;
Wolff, M ;
Watson, MH ;
Henze, M ;
Divita, G ;
Reed, SI .
NATURE STRUCTURAL BIOLOGY, 2001, 8 (05) :417-422
[8]   Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center [J].
Biggins, S ;
Ivanovska, I ;
Rose, MD .
JOURNAL OF CELL BIOLOGY, 1996, 133 (06) :1331-1346
[9]   Human immunodeficiency virus type 1 Vpr protein binds to the uracil DNA glycosylase DNA repair enzyme [J].
Bouhamdan, M ;
Benichou, S ;
Rey, F ;
Navarro, JM ;
Agostini, I ;
Spire, B ;
Camonis, J ;
Slupphaug, G ;
Vigne, R ;
Benarous, R ;
Sire, J .
JOURNAL OF VIROLOGY, 1996, 70 (02) :697-704
[10]  
Chang F, 2000, MICROSC RES TECHNIQ, V49, P161, DOI 10.1002/(SICI)1097-0029(20000415)49:2<161::AID-JEMT8>3.3.CO