In vitro evaluation of freeze-dried bone allografts combined with platelet rich plasma and human bone marrow stromal cells for tissue engineering

被引：34

作者：

Cenni, Elisabetta ^{[1
]}

Perut, Francesca ^{[1
]}

Ciapetti, Gabriela ^{[1
]}

Savarino, Lucia ^{[1
]}

Dallari, Dante ^{[2
]}

Cenacchi, Annarita ^{[3
]}

Stagni, Cesare ^{[2
]}

Giunti, Armando ^{[1
,2
]}

Fornasari, Pier Maria ^{[3
]}

Baldini, Nicola ^{[1
,2
]}

机构：

[1] Ist Ortoped Rizzoli, Lab Pathophysiol Orthopaed Implants, I-40136 Bologna, Italy

[2] Ist Ortoped Rizzoli, Dept Orthopaed Surg, I-40136 Bologna, Italy

[3] Ist Ortoped Rizzoli, Unit Immunohematol & Transfus Med, I-40136 Bologna, Italy

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2009年 / 20卷 / 01期

关键词：

FIBROBLAST-GROWTH-FACTOR; DEFECTS; GRAFTS; VIVO; PROLIFERATION; ENHANCEMENT; EXPRESSION; SCAFFOLDS;

D O I：

10.1007/s10856-008-3544-9

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Freeze-dried bone allograft (FDBA) might be more effective in combination with platelet rich plasma (PRP) and bone marrow stromal cells (BMSC) in accelerating bone healing. The isolation of BMSC through density gradient (pBMSC) is not extensively applicable in clinical practice, because it increases the risk of infection. Alternatively, BMSC can be concentrated by simple centrifugation (wBMSC) directly in the operating room. However, we do not know if wBMSC act in the same way as pBMSC. BMSC from 10 donors were tested whether, in the presence of a combination of FDBA and autologous PRP, the osteogenic differentiation of the cells concentrated by simple centrifugation (wBMSC + FDBA + PRP) was similar to that of pBMSC. Cell-associated alkaline phosphatase, osterix and fibroblast growth factor-2 were higher in wBMSC + FDBA + PRP. In conclusion, the combination of FDBA and PRP had a favouring effect on the differentiation towards osteoblasts and allowed BMSC concentrated by simple centrifugation to differentiate as fast as BMSC purified by density gradient.

引用

页码：45 / 50

页数：6

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