The production of soluble interleukin 4 receptors is preferentially regulated by the murine Th-2 cell subset

In order to understand item the endogenous production of soluble IL-4 receptors (sIL-4r) is regulated, the authors tested prototypic clones of Th-1 and Th-2 murine CD4(+) T cell subsets for their ability to regulate their expression of sIL-4r. Results showed that although both types of clones produced low levels of sIL-4r under resting conditions, only the Th-2 clones upregulated sIL-4r expression following antigenic stimulation, Inhibition of endogenous IL-4 with a neutralizing anti-IL-4 mAb had only a minor (similar to 20%) inhibitory effect on sIL-4r production by the Th-2 cells, and addition of rIL-4 to Th-1 cells resulted only in a modest two-fold increase in sIL-4r levels, suggesting that IL-4 is not the only factor that regulates sIL-4r production and that the ability of Th-2 clones to upregulate sIL-4r expression can be relatively independent of IL-4, Indeed, the production of sIL-4r by Thr cells was found to be regulated by cell contact and/or LL-I-mediated signals, Transcripts for both sIL-4r and mIL-4r were detected by RT-PCR on both resting and activated Th-1 and Th-2 cells, with the relative levels of expression being moderately higher in the Th-2 clones, Moreover, the expression of sIL-4r-specifie transcripts appeared to increase to a greater extent than those of mIL-4r after activation of Th-2 cells with APCs, both in the presence and absence of antigen, Taken together, these results predict that increased sIL-4r production in vivo might he preferentially associated with Th-2-type responses and indicate that even though the production of IL-4 and sIL-4r is mediated by the same cells (i.e. Th-2 cells), the synthesis of sIL-4r can be regulated independently from that of IL-4 through alternative signals such as cell contact and/or IL-l, These properties may allow for changing ratios of sIL-4r to IL-4 and sIL-4r to mIL-4r during different phases of an immune response and are consistent with a regulatory role for sIL-4r on IL-4 activity in vivo. (C) 1997 Academic Press Limited.