Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro

被引：56

作者：

Al Tanoury, Ziad ^{[1
,2
,3
]}

Rao, Jyoti ^{[2
,3
]}

Tassy, Olivier ^{[1
]}

Gobert, Benedicte ^{[1
,4
]}

Gapon, Svetlana ^{[2
]}

Garnier, Jean-Marie ^{[1
]}

Wagner, Erica ^{[2
]}

Hick, Aurore ^{[4
]}

Hall, Arielle ^{[5
]}

Gussoni, Emanuela ^{[5
]}

Pourquie, Olivier ^{[1
,2
,3
,6
]}

机构：

[1] Univ Strasbourg, Inst Genet & Biol Mol & Cellulaire IGBMC, Dept Dev & Stem Cells, CNRS,UMR 7104,Inserm U964, F-67404 Illkirch Graffenstaden, France

[2] Brigham & Womens Hosp, Dept Pathol, 60 Fenwood Rd, Boston, MA 02115 USA

[3] Harvard Med Sch, Dept Genet, 60 Fenwood Rd, Boston, MA 02115 USA

[4] Anagenesis Biotechnol, Parc Innovat BioParc 3,850 Blvd Sebastien Brandt, F-67400 Illkirch Graffenstaden, France

[5] Boston Childrens Hosp, Div Genet & Genom, 3 Blackfan Circle, Boston, MA 15021 USA

[6] Harvard Univ, Harvard Stem Cell Inst, Cambridge, MA 02138 USA

来源：

DEVELOPMENT | 2020年 / 147卷 / 12期

基金：

欧洲研究理事会; 美国国家卫生研究院;

关键词：

PAX7; Skeletal muscle; Human development; Pluripotent stem cell; Satellite cell; PLURIPOTENT STEM-CELLS; MUSCLE SATELLITE CELLS; SOMITIC ORIGIN; LIMB MUSCLE; PROGENITOR; POPULATION; MYOBLASTS; VIVO; HETEROGENEITY; PURIFICATION;

D O I：

10.1242/dev.187344

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Satellite cells (SC) are muscle stem cells that can regenerate adult muscles upon injury. Most SC originate from PAX7(+) myogenic precursors set aside during development. Although myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we report the generation of human induced pluripotent stem cell (iPSC) reporter lines in which fluorescent proteins have been introduced into the PAX7(+) and MYOG loci. We use single cell RNA sequencing to analyze the developmental trajectory of the iPSC-derived PAX7(+) myogenic precursors. We show that the PAX7(+) cells generated in culture can produce myofibers and self-renew in vitro and in vivo. Together, we demonstrate that cells exhibiting characteristics of human fetal satellite cells can be produced in vitro from iPSC, opening interesting avenues for muscular dystrophy cell therapy. This work provides significant insights into the development of the human myogenic lineage.

引用

页数：13

共 77 条

[1]

CD82 Is a Marker for Prospective Isolation of Human Muscle Satellite Cells and Is Linked to Muscular Dystrophies [J].