Zein accumulation in forage species (Lotus corniculatus and Medicago sativa) and co-expression of the γ-zein:KDEL and β-zein:: KDEL polypeptides in tobacco leaf

被引：13

作者：

Bellucci, M ^{[1
]}

Alpini, A ^{[1
]}

Arcioni, S ^{[1
]}

机构：

[1] CNR, IRMGPF, I-06128 Perugia, Italy

来源：

PLANT CELL REPORTS | 2002年 / 20卷 / 09期

关键词：

Medicago sativa; Lotus corniculatus; transgenic plants; sulphur-rich amino acids; zein;

D O I：

10.1007/s00299-001-0413-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plants of birdsfoot trefoil (Lotus corniculatus), alfalfa (Medicago sativa) and tobacco (Nicotiana tabacum) were transformed by Agrobacterium with binary vectors harbouring genes that code either for wild-type and gamma-zein:KDEL, or for beta-zein:KDEL. The maize seed storage proteins gamma-zein and beta-zein are rich in sulphur amino acids, and our long-term goal is to improve alfalfa and birdsfoot trefoil forage quality. Significant levels of zeins were detected in leaves of birdsfoot trefoil transformants, ranging up to 0.055% and 0.30% of total extractable protein for gamma-zein and beta-zein:KDEL, respectively. In leaves of alfalfa, the gamma-zein:KDEL expression level was up to 0.026% of the total extractable protein. From a F-1 population of transgenic tobacco, a plant was selected in which the amount of zeins (gamma-zein:KDEL plus beta-zein:KDEL) accounted for 1.1% of the total extractable protein.

引用

收藏

页码：848 / 856

页数：9

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