Induction of apoptosis in osteosarcoma s180 cells by polysaccharide from dictyophora indusiata

被引：34

作者：

Zhong, Biao ^{[1
]}

Ma, Yushui ^{[2
]}

Fu, Da ^{[2
]}

Zhang, Chi ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Orthopaed Surg, Shanghai Peoples Hosp 6, Shanghai 200030, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200030, Peoples R China

来源：

CELL BIOCHEMISTRY AND FUNCTION | 2013年 / 31卷 / 08期

基金：

中国国家自然科学基金;

关键词：

apoptosis; polysaccharide; Dictyophora indusiata; osteosarcoma; INHIBITION; ACTIVATION; GROWTH; P53;

D O I：

10.1002/cbf.2961

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Polysaccharides have shown great importance in cancer therapy. The current study showed that a polysaccharide from Dictyophora indusiata (PDI) also possessed anti-cancer properties. Methyl thiazolyl tetrazolium assay revealed a dose-dependent reduction of osteosarcoma S180 growth in response to PDI treatment. Apoptosis was observed following treatments with PDI, as reflected by the appearance of the subdiploid fraction and DNA fragmentations. We then investigated effects of PDI on expression of apoptosis-associated genes and the results revealed an increase of expression of bcl-2 and decreases of cdk4 and p53 protein levels. Finally, PDI treatment significantly increased the activation of caspase-3, a key executioner of apoptosis. These findings indicate that PDI may act as a chemopreventive and/or chemotherapeutic agent in osteosarcoma cells by reducing cell viability and inducing apoptosis. Copyright (c) 2013 John Wiley & Sons, Ltd.

引用

页码：719 / 723

页数：5

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