Jmjd2c histone demethylase enhances the expression of Mdm2 oncogene

被引：50

作者：

Ishimura, Akihiko ^{[1
]}

Terashima, Minoru ^{[1
]}

Kimura, Hiroshi ^{[2
]}

Akagi, Keiko ^{[3
]}

Suzuki, Yutaka ^{[4
]}

Sugano, Sumio ^{[4
]}

Suzuki, Takeshi ^{[1
]}

机构：

[1] Kanazawa Univ, Canc Res Inst, Div Funct Genom, Mol & Cellular Targeting Translat Oncol Ctr, Kanazawa, Ishikawa 9200934, Japan

[2] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

[3] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA

[4] Univ Tokyo, Grad Sch Frontier Sci, Kashiwa, Chiba 2778561, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 389卷 / 02期

关键词：

Histone demethylase; JmjC-domain; Oncogene; Transcription; p53; GENE; IDENTIFICATION; LYSINE-9; GASC1; P53;

D O I：

10.1016/j.bbrc.2009.08.155

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Jmjd2c is a candidate oncogene that encodes histone lysine demethylase. In this study, we discovered that over-expression of Jmjd2c increased the expression of Mdm2 oncogene dependent on its demethylase activity, which led to the reduction of p53 tumor suppressor gene product in the cells. A chromatin immunoprecipitation assay showed that Jmjd2c was recruited to the P2 promoter region of Mdm2 gene resulting in demethylation of histone H3 lysine 9, as typically found in actively transcribed genes. Furthermore, siRNA-mediated knockdown of Jmjd2c caused the reduction of Mdm2 expression in the cells. These results indicate that Mdm2 oncogene is a downstream target of Jmjd2c and may play an important role in Jmjd2c-mediated oncogenesis. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：366 / 371

页数：6

共 23 条

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