Molecular mapping and characterization of an RGA locus RGAPtokin1-2171 in chickpea

被引：26

作者：

Rajesh, PN ^{[1
]}

Tekeoglu, M

Gupta, VS

Ranjekar, PK

Muehlbauer, FJ

机构：

[1] Natl Chem Lab, Div Biochem Sci, Plant Mol Biol Unit, Pune 411008, Maharashtra, India

[2] Washington State Univ, USDA ARS, Pullman, WA 99164 USA

[3] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA

来源：

EUPHYTICA | 2002年 / 128卷 / 03期

关键词：

ascochyta blight resistance; bulk segregant analysis; resistance gene analog;

D O I：

10.1023/A:1021246600340

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Resistance gene analog polymorphism (RGAP) is a targeted homology based method, which has been used in different crops to identify tightly linked markers for disease resistance genes and also to enrich the map with a different class of markers. In chickpea, using the RGA primers, which are designed based on the conserved motifs present in characterized R-genes, Bulk Segregant Analysis (BSA) was performed on a resistant bulk and a susceptible bulk along with parents for ascochyta blight resistance. Of all available RGAs and their 48 different combinations, only one RGA showed polymorphism during BSA. This marker was evaluated in an F-7:8 population of 142 RILs from an interspecific cross of C. arietinum (FLIP 84-92C) x C. reticulatum ( PI 599072) and was mapped to Cicer linkage map. The genomic location of chickpea RGA was compared with the locations of mapped chickpea R-genes. This is the first RGA marker mapped to chickpea linkage map.

引用

页码：427 / 433

页数：7

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