Enhancement of growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by the addition of putrescine

被引：26

作者：

Wei, Ming ^{[1
]}

Jiang, Shao-Tong ^{[1
]}

Luo, Jian-Ping ^{[1
]}

机构：

[1] Hefei Univ Technol, Dept Biotechnol & Food Engn, Hefei 230009, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 03期

关键词：

Dendrobium huoshanense; polysaccharides; protocorm-like bodies; putrescine;

D O I：

10.1007/s10529-006-9248-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Putrescine at 0.6 mM stimulated protocorm-like body growth and polysaccharide synthesis in suspension cultures of Dendrobium huoshanense. The specific growth rate of protocorm-like body increased from 0.047 to 0.056 day(-1), and the maximum dry weight and polysaccharide production reached 33.2 and 2.94 g l(-1), respectively, while they were 24.6 and 2.12 g l(-1), respectively, in the control. The administration of polyamine inhibitor, alpha-DL-difluoromethylarginine, at 1 mM, decreased protocorm-like body growth and polysaccharide production to 21.4 and 1.76 g l(-1), respectively.

引用

页码：495 / 499

页数：5

共 13 条

[1]

Putrescine influences growth and production of coumarins in hairy root cultures of witloof chicory (Cichorium intybus L-cv. Luckuow local) [J].