Variability of soil microbial properties: Effects of sampling, handling and storage

被引:44
作者
Cernohlavkova, Jitka [1 ]
Jarkovsky, Jiri [1 ]
Negporova, Michala [1 ]
Hofman, Jakub [1 ]
机构
[1] Masaryk Univ, RECETOX Res Ctr Environm Chem & Ecotoxicol, Fac Sci, CZ-62500 Brno, Czech Republic
关键词
Soil biomonitoring; Microbial biomass; Respiration; Spatial variability; Sample storage; DRYING-REWETTING FREQUENCY; FREEZE-THAW CYCLES; NITROGEN MINERALIZATION; SPATIAL VARIABILITY; COLD-STORAGE; COMMUNITY STRUCTURE; CARBON; BIOMASS; MICROORGANISMS; DECREASES;
D O I
10.1016/j.ecoenv.2009.04.023
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
We investigated the effect of soil spatial variability within the sampling site scale, the effects of sample sieving (1, 2 and 4 mm), and storage conditions up to 32 weeks (wet at 4 degrees C, -20 degrees C and air dried) on microbial biomass C, respiration, ammonification and nitrification activities in arable, grassland and forest soil. In general, all results were dependent on soil type. Arable soil showed the highest spatial variability, followed by grassland and forest soil. Sieving did not cause large differences; however, higher biomass C and respiration activity were observed in the 1 mm than in the 4 mm fraction. Storage at 4 degrees C seemed to be the most appropriate up to 8 weeks showing only minor changes of microbial parameters. Freezing of soils resulted in large increase of respiration. Dried storage indicated disruption of microbial communities even after 2 weeks. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:2102 / 2108
页数:7
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