Phosphorus effects on the mycelium and storage structures of an arbuscular mycorrhizal fungus as studied in the soil and roots by analysis of fatty acid signatures

被引:167
作者
Olsson, PA [1 ]
Baath, E [1 ]
Jakobsen, I [1 ]
机构
[1] RISO NATL LAB, PLANT BIOL & BIOGEOCHEM DEPT, DK-4000 ROSKILDE, DENMARK
关键词
D O I
10.1128/AEM.63.9.3531-3538.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The distribution of an arbuscular mycorrhizal (AM) fungus between soil and roots, and between mycelial and storage structures, was studied by use of the fatty acid signature 16:1 omega 5. Increasing the soil phosphorus level resulted in a decrease in the level of the fatty acid 16:1 omega 5 in the soil and roots. A similar decrease was detected by microscopic measurements of root colonization and of the length of AM fungal hyphae in the soil. The fatty acid 16:1 omega 5 was estimated from two types of lipids, phospholipids and neutral lipids, which mainly represent membrane lipids and storage lipids, respectively. The numbers of spores of the AM fungus formed in the soil correlated most closely, with neutral lipid fatty acid 16:1 omega 5, whereas the hyphal length in the soil correlated most closely with phospholipid fatty acid 16:1 omega 5. The fungal neutral lipid/phospholipid ratio in the extraradical mycelium was positively correlated with the level of root infection and thus decreased with increasing applications of P. The neutral lipid/phospholipid ratio indicated that at high P levels, less carbon was allocated to storage structures. At all levels of P applied, the major part of the AM fungus was found to he present outside the roots, as estimated from phospholipid fatty acid 16:1 omega 5. The ratio of extraradical biomass/intraradical biomass was not affected by the application of P, except for a decrease at the highest level of P applied.
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页码:3531 / 3538
页数:8
相关论文
共 38 条
[1]   THE EFFECT OF PHOSPHORUS ON THE FORMATION OF HYPHAE IN SOIL BY THE VESICULAR ARBUSCULAR MYCORRHIZAL FUNGUS, GLOMUS-FASCICULATUM [J].
ABBOTT, LK ;
ROBSON, AD ;
DEBOER, G .
NEW PHYTOLOGIST, 1984, 97 (03) :437-446
[2]  
ARNEBRANT K, 1994, MYCORRHIZA, V5, P7, DOI 10.1007/BF00204014
[3]   THE EFFECT OF ADDED NITROGEN AND PHOSPHORUS ON MYCORRHIZAL GROWTH-RESPONSE AND INFECTION IN ALLIUM-SCHOENOPRASUM [J].
BAATH, E ;
SPOKES, J .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1989, 67 (11) :3227-3232
[5]   STABILITY AND HERITABILITY OF FATTY-ACID METHYL-ESTER PROFILES OF GLOMALEAN ENDOMYCORRHIZAL FUNGI [J].
BENTIVENGA, SP ;
MORTON, JB .
MYCOLOGICAL RESEARCH, 1994, 98 :1419-1426
[6]   COMPARISON OF 2 METHODS FOR QUANTIFYING EXTRARADICAL MYCELIUM OF VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI [J].
BETHLENFALVAY, GJ ;
AMES, RN .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1987, 51 (03) :834-837
[7]   RELATIONSHIPS BETWEEN HOST AND ENDOPHYTE DEVELOPMENT IN MYCORRHIZAL SOYBEANS [J].
BETHLENFALVAY, GJ ;
BROWN, MS ;
PACOVSKY, RS .
NEW PHYTOLOGIST, 1982, 90 (03) :537-543
[8]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[9]   LIPID PHYSIOLOGY OF VESICULAR-ARBUSCULAR MYCORRHIZA .1. COMPOSITION OF LIPIDS IN ROOTS OF ONION, CLOVER AND RYEGRASS INFECTED WITH GLOMUS-MOSSEAE [J].
COOPER, KM ;
LOSEL, DM .
NEW PHYTOLOGIST, 1978, 80 (01) :143-+
[10]   THE EFFECT OF SOIL-PHOSPHORUS ON THE EXTERNAL MYCELIUM GROWTH OF ARBUSCULAR MYCORRHIZAL FUNGI DURING THE EARLY STAGES OF MYCORRHIZA FORMATION [J].
DEMIRANDA, JCC ;
HARRIS, PJ .
PLANT AND SOIL, 1994, 166 (02) :271-280