A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

被引：86

作者：

De Antoni, A ^{[1
]}

Gallwitz, D ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Mol Genet, D-37070 Gottingen, Germany

来源：

GENE | 2000年 / 246卷 / 1-2期

关键词：

Cre-recombinase; G418; resistance; HA; PCR-tagging; loxP; MYC; polyhistidine; VSV;

D O I：

10.1016/S0378-1119(00)00083-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Gene tagging can be achieved by homologous recombination in yeast. The kan(t) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：179 / 185

页数：7

共 15 条

[1]

Ausubel F.M., 1996, CURRENT PROTOCOLS MO

[2]

Goldstein AL, 1999, YEAST, V15, P507, DOI 10.1002/(SICI)1097-0061(199904)15:6<507::AID-YEA369>3.0.CO

[3]

2-P

[4] A new efficient gene disruption cassette for repeated use in budding yeast [J].

Guldener, U ;

Heck, S ;

Fiedler, T ;

Beinhauer, J ;

Hegemann, JH .

NUCLEIC ACIDS RESEARCH, 1996, 24 (13) :2519-2524

[5]

Knop M, 1999, YEAST, V15, P963, DOI 10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO

[6]

2-W

[7]

Longtine MS, 1998, YEAST, V14, P953, DOI 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.3.CO

[8]

2-L

[9] GENE DISRUPTION WITH PCR PRODUCTS IN SACCHAROMYCES-CEREVISIAE [J].

LORENZ, MC ;

MUIR, RS ;

LIM, E ;

MCELVER, J ;

WEBER, SC ;

HEITMAN, J .

GENE, 1995, 158 (01) :113-117

[10] FUNCTIONAL EXPRESSION OF THE CRE-LOX SITE-SPECIFIC RECOMBINATION SYSTEM IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].

SAUER, B .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (06) :2087-2096

← 1 2 →