A physical assay for meiotic recombination in Coprinus cinereus

被引：2

作者：

Freedman, T ^{[1
]}

Pukkila, PJ ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT BIOL,CHAPEL HILL,NC 27599

来源：

MOLECULAR & GENERAL GENETICS | 1997年 / 254卷 / 04期

基金：

美国国家科学基金会;

关键词：

meiotic recombination; polymerase chain reaction; Coprinus cinereus;

D O I：

10.1007/s004380050429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used the polymerase chain reaction (PCR) method to monitor meiotic recombination in the basidiomycete Coprinus cinereus. We used DNA-mediated transformation to recover strains with modifications of the trp1 locus. The modifications were designed to introduce unique PCR priming sites separated by a homologous 2.4 kb region in which crossing over could occur. We showed that exchange occurred in this region at the frequency expected for a typical region of this genome (2.4 kb should correspond to a genetic length of 0.08 cM). We also detected products resulting from crossing over in DNAs extracted from cells in meiotic prophase. The assay should be useful for monitoring exchange in mutants that cannot complete meiosis.

引用

页码：372 / 378

页数：7

共 26 条

[1] PCR AMPLIFICATION OF UP TO 35-KB DNA WITH HIGH-FIDELITY AND HIGH-YIELD FROM LAMBDA-BACTERIOPHAGE TEMPLATES [J].