Astragalus polysaccharide improves muscle atrophy from dexamethasone- and peroxide-induced injury in vitro

被引：67

作者：

Lu, Lu ^{[1
,2
]}

Wang, Dong-Tao ^{[1
,2
]}

Shi, Ying ^{[1
,2
]}

Yin, Yi ^{[1
,2
]}

Wei, Lian-Bo ^{[1
,2
]}

Zou, Yu-Cong ^{[1
]}

Huang, Bo ^{[3
]}

Zhao, Yan ^{[3
]}

Wang, Ming ^{[1
]}

Wan, Heng ^{[4
]}

Li, Cheng-Jie ^{[1
,2
]}

Diao, Jian-Xin ^{[2
]}

机构：

[1] Southern Med Univ, ZhuJiang Hosp, Dept Tradit Chinese Med, Guangzhou 510280, Guangdong, Peoples R China

[2] Southern Med Univ, Sch Tradit Chinese Med, Guangzhou 510515, Guangdong, Peoples R China

[3] Southern Med Univ, ZhuJiang Hosp, Dept Nephrol, Guangzhou 510280, Guangdong, Peoples R China

[4] Southern Med Univ, Affiliated Hosp 3, Dept Endocrinol, Guangzhou 510630, Guangdong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 61卷

基金：

美国国家科学基金会;

关键词：

Astragalus polysaccharide; Atrophy; Apoptosis; Myotubes; Myoblast; UBIQUITIN-PROTEASOME PATHWAY; GROWTH-FACTOR-I; NF-KAPPA-B; SKELETAL-MUSCLE; TRANSCRIPTION FACTORS; MITOCHONDRIAL ROS; SIGNALING PATHWAY; C2C12; MYOTUBES; CELL-SURVIVAL; APOPTOSIS;

D O I：

10.1016/j.ijbiomac.2013.06.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Astragalus polysaccharide (APS) is an important bioactive component of Astragalus membranaceus Bunge (Leguminosae) that has been used in traditional Chinese medicine for treating muscle wasting, a serious complication with complex mechanism manifested as myofibers atrophy and satellite cells apoptosis. In this study, the anti-atrophy and anti-apoptotic activity of Astragalus polysaccharide (APS) was characterized in C2C12 skeletal muscle myotubes and myoblasts. APS inhibited dexamethasone-induced atrophy by restoring phosphorylation of Akt, m-TOR, P70s6k, rpS6 and FoxO3A/FoxO1. The targets that protected C2C12 myoblasts from damage by H2O2 were promoting cells proliferation and inhibiting cells apoptosis. The protective mechanisms involved mitochondrial pathway and death receptor pathway. Moreover, Antioxidant effect of APS was also detected in this work. Our findings suggested that APS could be explored as a protective and perhaps as a therapeutic agent in the management of muscle wasting. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：7 / 16

页数：10

共 54 条

[1]

Astragalus saponins modulate mTOR and ERK signaling to promote apoptosis through the extrinsic pathway in HT-29 colon cancer cells [J].