The transport of nanosized gene carriers unraveled by live-cell imaging

被引：101

作者：

Bausinger, R

von Gersdorff, K

Braeckmans, K

Ogris, M

Wagner, E

Bräuchle, C

Zumbusch, A

机构：

[1] Univ Munich, Dept Chem & Biochem, D-81377 Munich, Germany

[2] Univ Munich, Ctr NanoSci, D-81377 Munich, Germany

[3] Univ Munich, Dept Pharm, D-81377 Munich, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 10期

关键词：

DNA; fluorescence microscopy; gene expression; intracellular transport; nanoparticles;

D O I：

10.1002/anie.200503021

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tracking transport: Cationic polyethylenimine nanoparticles serve as nonviral vectors and deliver DNA to the nucleus of cells. Fluorescence live-cell imaging highlights the interactions between these polyplexes and the cytoskeleton during the subsequent stages oftransfection (see picture). The analysis of the underlying transport processes gives new impulse to further chemical-synthesis approaches. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1568 / 1572

页数：5

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