Is the mammalian porin channel, VDAC, a perfect cylinder in the high conductance state?

被引：19

作者：

Carneiro, CMM

Krasilnikov, OV

Yuldasheva, LN

deCarvalho, ACC

Nogueira, RA

机构：

[1] INST PHYSIOL & BIOPHYS,MOL PHYSIOL LAB,TASHKENT 700095,UZBEKISTAN

[2] UNIV FED PERNAMBUCO,DEPT BIOPHYS & RADIOBIOL,CTR BIOL SCI,MEMBRANE BIOPHYS LAB,BR-50670901 RECIFE,PE,BRAZIL

[3] TASHKENT PEDIAT MED INST,DEPT BIOCHEM,TASHKENT 700125,UZBEKISTAN

[4] UNIV FED RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,BR-21941 RIO JANEIRO,BRAZIL

来源：

FEBS LETTERS | 1997年 / 416卷 / 02期

关键词：

voltage-dependent anion-selective channel; ion channel; size;

D O I：

10.1016/S0014-5793(97)01198-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The mammalian porin channel (VDAC, porin-31BM) mas reconstituted in planar lipid bilayers under voltage clamp conditions, The radii of both entrances of the channel were examined using a method that consisted in filling the channel with different non-electrolytes through its cis or trans entrances while recording single channel conductances, As a result it was found that the geometry of channels formed by porin-31BM could not be approximated by a perfectly cylindrical pore, In fact there is an asymmetry in the geometry of the channel: the diameters of the cis and trans entrances were estimated to be similar to 2 nm and similar to 4 nm respectively. (C) 1997 Federation of European Biochemical Societies.

引用

页码：187 / 189

页数：3

共 19 条

[1]

PERMEATION OF HYDROPHILIC SOLUTES THROUGH MITOCHONDRIAL OUTER MEMBRANES - REVIEW ON MITOCHONDRIAL PORINS [J].