Single-dose, virus-vectored vaccine protection against Yersinia pestis challenge: CD4+ cells are required at the time of challenge for optimal protection

被引：25

作者：

Chattopadhyay, Anasuya ^{[1
]}

Park, Steven ^{[2
]}

Delmas, Guillaume ^{[2
]}

Suresh, Rema ^{[2
]}

Senina, Svetlana ^{[2
]}

Perlin, David S. ^{[2
]}

Rose, John K. ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06510 USA

[2] Publ Hlth Res Inst, Newark, NJ 07103 USA

来源：

VACCINE | 2008年 / 26卷 / 50期

关键词：

Yersinia pestis; Vesicular stomatitis virus; CD4(+) cells;

D O I：

10.1016/j.vaccine.2008.09.031

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

We have developed an experimental recombinant vesicular stomatitis virus (VSV) vectored plague vaccine expressing a secreted form of Yersinia pestis low calcium response protein V (LcrV) from the first position of the VSV genome. This vector, given intramuscularly in a single dose, induced high-level antibody titers to LcrV and gave 90-100% protection against pneumonic plague challenge in mice. This single-dose protection was significantly better than that generated by VSV expressing the non-secreted LcrV protein. Increased protection correlated with increased anti-LcrV antibody and a bias toward IgG2a and away from IgG1 isotypes. We also found that the depletion of CD4(+) cells, but not CD8(+) cells, at the time of challenge resulted in reduced vaccine protection, indicating a role for cellular immunity in protection. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：6329 / 6337

页数：9

共 63 条

[1]

Recombinant V antigen protects mice against pneumonic and bubonic plague caused by F1-capsule-positive and -negative strains of Yersinia pestis [J].

Anderson, GW ;

Leary, SEC ;

Williamson, ED ;

Titball, RW ;

Welkos, SL ;

Worsham, PL ;

Friedlander, AM .

INFECTION AND IMMUNITY, 1996, 64 (11) :4580-4585

[2]

Development of in vitro correlate assays of immunity to infection with Yersinia pestis [J].

Bashaw, J. ;

Norris, S. ;

Weeks, S. ;

Trevino, S. ;

Adamovicz, J. J. ;

Welkos, S. .

CLINICAL AND VACCINE IMMUNOLOGY, 2007, 14 (05) :605-616

[3]

Benner GE, 1999, INFECT IMMUN, V67, P1922

[4]

Protective immunity against respiratory tract challenge with Yersinia pestis in mice immunized with an adenovirus-based vaccine vector expressing V antigen [J].

Chiuchiolo, Maria J. ;

Boyer, Julie L. ;

Krause, Anja ;

Senina, Svetlana ;

Hackett, Neil R. ;

Crystal, Ronald G. .

JOURNAL OF INFECTIOUS DISEASES, 2006, 194 (09) :1249-1257

[5]

DIETZSCHOLD B, 1996, FIELDS VIROLOGY, P1137

[6]

Broad T cell immunity to the LcrV virulence protein is induced by targeted delivery to DEC-205/CD205-positive mouse dendritic cells [J].

Do, Yoonkyung ;

Park, Chae Gyu ;

Kang, Young-Sun ;

Park, Sung Ho ;

Lynch, Rebecca M. ;

Lee, Haekyung ;

Powell, Bradford S. ;

Steinman, Ralph M. .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2008, 38 (01) :20-29

[7]

Immunogenicity of attenuated vesicular stomatitis virus vectors expressing HIV type 1 env and SIV Gag proteins: Comparison of intranasal and intramuscular vaccination routes [J].

Egan, MA ;

Chong, SY ;

Rose, NF ;

Megati, S ;

Lopez, KJ ;

Schadeck, EB ;

Johnson, JE ;

Masood, A ;

Piacente, P ;

Druilhet, RE ;

Barras, PW ;

Hasselschwert, DL ;

Reilly, P ;

Mishkin, EM ;

Montefiori, DC ;

Lewis, MG ;

Clarke, DK ;

Hendry, RM ;

Marx, PA ;

Eldridge, JH ;

Udem, SA ;

Israel, ZR ;

Rose, JK .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 2004, 20 (09) :989-1004

[8]

Stat 4 but not Stat 6 mediated immune mechanisms are essential in protection against plague [J].

Elvin, SJ ;

Williamson, ED .

MICROBIAL PATHOGENESIS, 2004, 37 (04) :177-184

[9]

Protection studies following bronchopulmonary and intramuscular immunisation with Yersinia pestis F1 and V subunit vaccines coencapsulated in biodegradable microspheres:: a comparison of efficacy [J].

Eyles, JE ;

Williamson, ED ;

Spiers, ID ;

Alpar, HO .

VACCINE, 2000, 18 (28) :3266-3271

[10]

EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].

FUERST, TR ;

NILES, EG ;

STUDIER, FW ;

MOSS, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126

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