The sodium ions inside a lipophilic G-quadruplex channel as probed by solid-state 23Na NMR

被引:58
作者
Wong, A
Fettinger, JC
Forman, SL
Davis, JT
Wu, G [1 ]
机构
[1] Queens Univ, Dept Chem, Kingston, ON K7L 3N6, Canada
[2] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
关键词
D O I
10.1021/ja0120330
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report solid-state 23Na NMR and X-ray crystallographic results for a self-assembled G-quadruplex channel formed by a guanine nucleoside, 5′-tert-butyl-dimethylsilyl-2′,3′-O-isopropylidene guanosine (G 1). The study provides an unambiguous 23Na NMR identification for the Na+ ions inside a lipophilic G-quadruplex channel. The crystalline nature of the sample yields a remarkably high resolution in the 23Na multiple-quantum magic-angle spinning (MQMAS) spectrum, making it possible to extract very accurate 23Na NMR parameters for each of the three crystallographically distinct Na sites. The observation of a single Na+ ion from a 9-kDa system demonstrates the potential of solid-state 23Na NMR as a complementary technique to X-ray for detecting Na+ ions in biological structures. Copyright © 2002 American Chemical Society.
引用
收藏
页码:742 / 743
页数:2
相关论文
共 25 条
  • [1] Z filtering in MQMAS NMR
    Amoureux, JP
    Fernandez, C
    Steuernagel, S
    [J]. JOURNAL OF MAGNETIC RESONANCE SERIES A, 1996, 123 (01) : 116 - 118
  • [2] Direct detection of monovalent metal ion binding to a DNA G-quartet by 205Tl NMR
    Basu, S
    Szewczak, AA
    Cocco, M
    Strobel, SA
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (13) : 3240 - 3241
  • [3] DETERMINATION OF THE CORRELATION TIME FOR A SLOWLY REORIENTING SPIN-3/2 NUCLEUS - BINDING OF NA+ WITH THE 5'-GMP SUPRAMOLECULAR ASSEMBLY
    DELVILLE, A
    DETELLIER, C
    LASZLO, P
    [J]. JOURNAL OF MAGNETIC RESONANCE, 1979, 34 (02) : 301 - 315
  • [4] Kinetics of sodium ion binding to DNA quadruplexes
    Deng, H
    Braunlin, WH
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1996, 255 (03) : 476 - 483
  • [5] ROLE OF ALKALI-METAL AND AMMONIUM CATIONS IN THE SELF-ASSEMBLY OF THE 5'-GUANOSINE MONOPHOSPHATE DIANION
    DETELLIER, C
    LASZLO, P
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1980, 102 (03) : 1135 - 1141
  • [6] Toward artificial ion channels: A lipophilic G-quadruplex
    Forman, SL
    Fettinger, JC
    Pieraccini, S
    Gottareli, G
    Davis, JT
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (17) : 4060 - 4067
  • [7] ISOTROPIC SPECTRA OF HALF-INTEGER QUADRUPOLAR SPINS FROM BIDIMENSIONAL MAGIC-ANGLE-SPINNING NMR
    FRYDMAN, L
    HARWOOD, JS
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (19) : 5367 - 5368
  • [8] Multistranded DNA structures
    Gilbert, DE
    Feigon, J
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1999, 9 (03) : 305 - 314
  • [9] Gottarelli G., 1996, COMPREHENSIVE SUPRAM, V9, P483
  • [10] 4-STRANDED NUCLEIC-ACID STRUCTURES 25 YEARS LATER - FROM GUANOSINE GELS TO TELOMER DNA
    GUSCHLBAUER, W
    CHANTOT, JF
    THIELE, D
    [J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1990, 8 (03) : 491 - 511