Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry

被引：25

作者：

ImbertMarcille, BM

Robillard, N

Poirier, AS

CosteBurel, M

Cantarovich, D

Milpied, N

Billaudel, S

机构：

[1] NANTES UNIV HOSP,VIROL LAB,F-44035 NANTES 01,FRANCE

[2] NANTES UNIV HOSP,FLOW CYTOMETRY DEPT,F-44035 NANTES 01,FRANCE

[3] NANTES UNIV HOSP,INST BIOL,F-44035 NANTES 01,FRANCE

[4] NANTES UNIV HOSP,INST TRANSPLANTAT & RES TRANSPLANTAT,F-44035 NANTES 01,FRANCE

[5] NANTES UNIV HOSP,DEPT CLIN HAEMATOL,F-44035 NANTES 01,FRANCE

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 10期

关键词：

D O I：

10.1128/JCM.35.10.2665-2669.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.

引用

页码：2665 / 2669

页数：5

共 22 条

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[2]

ELMENDORF S, 1988, CYTOMETRY, V9, P254

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