Isolation of RNA from blackcurrant (Ribes nigrum L) fruit

被引：25

作者：

Woodhead, M ^{[1
]}

Taylor, MA ^{[1
]}

Davies, HV ^{[1
]}

Brennan, RM ^{[1
]}

McNicol, RJ ^{[1
]}

机构：

[1] SCOTTISH CROP RES INST,DEPT CELLULAR & ENVIRONM PHYSIOL,DUNDEE DD2 5DA,SCOTLAND

来源：

MOLECULAR BIOTECHNOLOGY | 1997年 / 7卷 / 01期

关键词：

RNA isolation; blackcurrant fruit; Ribes nigrum;

D O I：

10.1007/BF02821541

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction.

引用

页码：1 / 4

页数：4

共 14 条

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