Isolation and characterization of four ascorbate peroxidase cDNAs responsive to water deficit in cowpea leaves

被引:75
作者
D'Arcy-Lameta, A
Ferrari-Iliou, R
Contour-Ansel, D
Pham-Thi, AT
Zuily-Fodil, Y
机构
[1] Univ Paris 12, Lab Ecophysiol Mol, UMR 137 Biosol, F-94010 Creteil, France
[2] Univ Paris 07, Lab Geochim Eaux, F-75251 Paris, France
[3] IPGP, F-75251 Paris, France
关键词
active oxygen species; drought tolerance; ascorbate peroxidase; abscissic acid; gene expression; Vigna unguiculata;
D O I
10.1093/aob/mcj010
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
center dot Background and Aims Abiotic stresses stimulate formation of active oxygen species in plant tissues. Among antioxidant mechanisms, H2O2 detoxication by ascorbate peroxidases (APX) plays an important role. Several APX isoforms exist in plant cells, and they have rarely been studied separately. The aim of this work was to study changes in cytosolic, peroxisomal, stromatic and thylakoid APX gene expression in response to progressive drought, rapid desiccation and application of exogenous abscisic acid in the leaves of cowpea (Vigna unguiculata) plants. center dot Methods Two cowpea (V. unguiculata) cultivars, 'EPACE-1' which is drought-tolerant and '1183'which is drought-sensitive, were submitted to drought stress by withholding irrigation. Detached leaves were air-dried or treated with exogenous abscisic acid. APX cDNAs were isolated by PCR and cloned in plasmid vectors. Changes in gene expression were studied using reverse-transcription PCR. center dot Key Results Four new V. unguiculata cDNAs encoding putative cytosolic, peroxisomal and chloroplastic (stromatic and thylakoidal) APX were isolated and characterized. In response to the different treatments, higher increases in steady-state transcript levels of the cytoplasmic and peroxisomal APX genes were observed in '1183' compared with 'EPACE-1'. On the other hand, the expression of the chloroplastic APX genes was stimulated earlier in the tolerant cultivar when submitted to progressive drought. center dot Conclusions Water deficit induced differences in transcript accumulation of APX genes between the two cultivars that were related to their respective tolerance to drought. Chloroplastic APX genes responded early to progressive water deficit in the tolerant plant, suggesting a capacity to efficiently detoxify active oxygen species at their production site. The more sensitive '1183' was also able to respond to drought by activating its whole set of APX genes.
引用
收藏
页码:133 / 140
页数:8
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