Regulation of human FcεRI α-chain gene expression by multiple transcription factors

被引：67

作者：

Nishiyama, C

Hasegawa, M

Nishiyama, M

Takahashi, K

Akizawa, Y

Yokota, T

Okumura, K

Ogawa, H

Ra, C

机构：

[1] Juntendo Univ, Sch Med, Allergy Res Ctr, Dept Immunol Bunkyo Ku, Tokyo 1138421, Japan

[2] Juntendo Univ, Sch Med, Allergy Res Ctr, Dept Dermatol Bunkyo Ku, Tokyo 1138421, Japan

[3] Univ Tokyo, Biotechnol Res Ctr, Tokyo, Japan

[4] Foods & Pharmaceut Res & Dev Lab, Ibaraki, Japan

来源：

JOURNAL OF IMMUNOLOGY | 2002年 / 168卷 / 09期

关键词：

D O I：

10.4049/jimmunol.168.9.4546

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Transcriptional regulation of the gene-encoding human FcepsilonRI alpha-chain was analyzed in detail. EMSA revealed that either YY1 or PU.1 bound to the region close to that recognized by Elf-1. The alpha-chain promoter activity was up-regulated similar to2-fold by exogenously expressed YY1 or PU.1 and similar to7-fold by GATA-1, respectively, in KU812 cells. In contrast, coexpression of GATA-1 with either of PU.1 or YY1 dramatically activated the promoter similar to41- or similar to27-fold, respectively. Especially synergic activation by GATA-1 and PU.1 was surprising, because these transcription factors are known to inhibit the respective transactivating activities of each other. These up-regulating effects of PU.1 and YY1 with GATA-1 were inhibited by overexpression of Elf-1, indicating that Elf-1 serves as a repressor for the alpha-chain gene expression. Transcriptional regulation of the alpha-chain gene through four transcriptional factors is discussed.

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页码：4546 / 4552

页数：7

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