Determination of leukotriene E4 in human urine using liquid chromatography-tandem mass spectrometry

被引：22

作者：

Mizugaki, M ^{[1
]}

Hishinuma, T ^{[1
]}

Suzuki, N ^{[1
]}

机构：

[1] Tohoku Univ Hosp, Dept Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808574, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 729卷 / 1-2期

关键词：

leukotrienes;

D O I：

10.1016/S0378-4347(99)00174-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A Liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E-4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg. to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6+/-2.4%. We detected LTE4 (63.1+/-18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：279 / 285

页数：7

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