Peptide synthesis by surfactant-chymotrypsin complexes in organic media

被引：5

作者：

Abe, K ^{[1
]}

Kawazoe, T ^{[1
]}

Okazaki, SY ^{[1
]}

Goto, M ^{[1
]}

Nakashio, F ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC ENGN,DEPT CHEM SCI & TECHNOL,FUKUOKA 81281,JAPAN

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 01期

关键词：

D O I：

10.1007/BF02764446

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide synthesis by surfactant-chymotrypsin (CT) complexes was performed in organic media containing 1%-8% (v/v) water. The CT complex exhibited a higher enzymatic activity than native CT. The control of the water content in the reaction media has a crucial effect on the enzymatic activity. The maximum conversion (57%) for the peptide synthesis by the surfactant-CT complex was obtained at 4% water content.

引用

页码：25 / 29

页数：5

共 8 条

[1] ENZYMATIC-SYNTHESIS OF X-PHE-LEU-NH2 IN LOW WATER-CONTENT SYSTEMS - INFLUENCE OF THE N-ALPHA PROTECTING GROUP AND THE REACTION MEDIUM COMPOSITION [J].

CALVET, S ;

CLAPES, P ;

TORRES, JL ;

VALENCIA, G ;

FEIXAS, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1164 (02) :189-196

[2] PREPARATIVE PRODUCTION OF OPTICALLY-ACTIVE ESTERS AND ALCOHOLS USING ESTERASE-CATALYZED STEREOSPECIFIC TRANS-ESTERIFICATION IN ORGANIC MEDIA [J].

CAMBOU, B ;

KLIBANOV, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (09) :2687-2692

[3] DEVELOPMENT OF NEW SURFACTANT FOR LIQUID SURFACTANT MEMBRANE PROCESS [J].

GOTO, M ;

MATSUMOTO, M ;

KONDO, K ;

NAKASHIO, F .

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1987, 20 (02) :157-164

[4] ENZYMATIC ESTERIFICATION BY SURFACTANT-COATED LIPASE IN ORGANIC MEDIA [J].

GOTO, M ;

KAMIYA, N ;

MIYATA, M ;

NAKASHIO, F .

BIOTECHNOLOGY PROGRESS, 1994, 10 (03) :263-268

[5]

GOTO M, 1995, BIOTECHNOL TECH, V9, P101

[6] APPLICATION OF POLYETHYLENE GLYCOL-MODIFIED ENZYMES IN BIOTECHNOLOGICAL PROCESSES - ORGANIC SOLVENT-SOLUBLE ENZYMES [J].

INADA, Y ;

TAKAHASHI, K ;

YOSHIMOTO, T ;

AJIMA, A ;

MATSUSHIMA, A ;

SAITO, Y .

TRENDS IN BIOTECHNOLOGY, 1986, 4 (07) :190-194

[7] ENANTIOSPECIFICITY OF ALPHA-CHYMOTRYPSIN AT S1' SITE FOR PEPTIDE-SYNTHESIS IN AQUEOUS-ORGANIC MEDIA [J].

KISE, H ;

NAGASHIMA, T .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1993, 66 (12) :3693-3698

[8] PEPTIDE-SYNTHESIS BY PROTEASES IN ORGANIC-SOLVENTS - MEDIUM EFFECT ON SUBSTRATE-SPECIFICITY [J].

NAGASHIMA, T ;

WATANABE, A ;

KISE, H .

ENZYME AND MICROBIAL TECHNOLOGY, 1992, 14 (10) :842-847

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